{"id":1861,"date":"2013-08-12T10:38:25","date_gmt":"2013-08-11T21:38:25","guid":{"rendered":"http:\/\/microbiologymatters.com\/?p=1861"},"modified":"2013-08-13T09:54:49","modified_gmt":"2013-08-12T20:54:49","slug":"should-anaerobes-be-cultured-from-superficial-swabs","status":"publish","type":"post","link":"https:\/\/microbiologymatters.com\/?p=1861","title":{"rendered":"&#8220;Should anaerobes be cultured from superficial swabs?&#8221;"},"content":{"rendered":"<p><span style=\"color: #0000ff;\">Anaerobic culture has always been difficult in a diagnostic bacteriology laboratory. In the early days\u00a0 of bacteriology labs anaerobic culture was very difficult if not impossible, so it just wasn\u2019t done.<\/span><\/p>\n<figure id=\"attachment_1863\" aria-describedby=\"caption-attachment-1863\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/i0.wp.com\/microbiologymatters.com\/wp-content\/uploads\/2013\/08\/800px-Bacteroides_fragilis_on_Fastidious_Anaerobe_Agar_-_Sensitive_to_Metronidazole_-_Detail.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-1863\" alt=\"Photograph taken by Nathan Reading\" src=\"https:\/\/i0.wp.com\/microbiologymatters.com\/wp-content\/uploads\/2013\/08\/800px-Bacteroides_fragilis_on_Fastidious_Anaerobe_Agar_-_Sensitive_to_Metronidazole_-_Detail-300x225.jpg?resize=300%2C225\" width=\"300\" height=\"225\" data-recalc-dims=\"1\" \/><\/a><figcaption id=\"caption-attachment-1863\" class=\"wp-caption-text\">Photograph taken by Nathan Reading<\/figcaption><\/figure>\n<p><span style=\"color: #0000ff;\">Then came along anaerobic jars and anaerobic cabinets where the oxygen could be removed from the environment surrounding the agar plate. This led to an &#8220;explosion&#8221; in anaerobic culture in many different types of samples.<\/span><\/p>\n<p><span style=\"color: #0000ff;\">Now the reality is starting to sink home&#8230; <span style=\"color: #ff0000;\">We are still not that good at recovering anaerobes.<\/span> This is not particularly the laboratory\u2019s fault. It is just very difficult to mimic the anaerobic conditions of\u00a0certain parts of the body right from when the bacteria are taken from the patient until they are growing in an anaerobic environment in the laboratory.\u00a0<\/span><\/p>\n<p><span style=\"color: #0000ff;\">In addition there are areas of the body such as the GI tract, vagina, oropharynx\u00a0which contain lots of anaerobes anyway, so culturing anaerobes from these areas means nothing at all in terms of pathogenicity.<\/span><\/p>\n<p><span style=\"color: #0000ff;\">So back to the question: <span style=\"color: #ff0000;\">Should superficial swabs be cultured for anaerobes?<\/span><\/span><\/p>\n<p><span style=\"color: #0000ff;\">My gut feeling here is that we are not helping the patient by culturing superficial swabs for anaerobes because:<\/span><\/p>\n<ul>\n<li><span style=\"color: #0000ff;\">Superficial areas are exposed to oxygen, so although anaerobes may exist in deeper areas of a wound, <span style=\"color: #ff0000;\">getting a good sample of such with a swab is very difficult.<\/span><\/span><\/li>\n<li><span style=\"color: #0000ff;\">\u00a0A clinician&#8217;s\u00a0 decision whether to include anaerobic\u00a0 cover should very much be decided by the clinical presentation, not the microbiological results. Eg bite wounds, infected diabetic foot ulcers, aspiration pneumonias, dental infections are all clinical conditions where anaerobic cover should generally be routine. <span style=\"color: #ff0000;\">Not culturing anaerobes from such specimens should not prompt discontinuation of anaerobic cover.<\/span><\/span><\/li>\n<li><span style=\"color: #0000ff;\">The growth of anaerobes or mixed anaerobes from superficial wound is of dubious value. If isolated, are the anaerobes really causing the problem? My experience is that when anaerobes are found in superficial swab culture, they often co-exist with other pathogens such as Staphylococcus aureus, <span style=\"color: #ff0000;\">and treatment of the Staph aureus alone often sorts out the infection.<\/span><\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #0000ff;\">There is no question that anaerobic culture is of more importance when the sample is taken from a sterile site. In these cases it can have a real impact on both the diagnosis and subsequent management of the patient. E.g. Fusobacterium necrophorum in a blood culture suggests the possibility of a Lemiere\u2019s syndrome. Isolating Bacteroides in a blood culture suggests intra-abdominal pathology, often a collection of some sort.<\/span><\/p>\n<p><span style=\"color: #0000ff;\">Lots of laboratories still culture for anaerobes from at least some superficial swabs, because that is the way it has always been, or that is what is expected of the lab, or because we\u00a0<span style=\"color: #ff0000;\">do<\/span>\u00a0isolate anaerobes from superficial swabs.<\/span><\/p>\n<p><span style=\"color: #0000ff;\">However I think that when we decide to culture a superficial swab for anaerobes, although we are trying to be helpful, the truth is that we may not be helping at all\u2026.<\/span><span style=\"color: #0000ff;\">\u00a0<\/span><\/p>\n<p>Michael<\/p>\n<p><em>p.s. Please let me know if any of the links on the articles are outdated and no longer work. I came across one tonight&#8230;!<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Anaerobic culture has always been difficult in a diagnostic bacteriology laboratory. In the early days\u00a0 of bacteriology labs anaerobic culture was very difficult if not impossible, so it just wasn\u2019t done. Then came along anaerobic jars and anaerobic cabinets where the oxygen could be removed from the environment surrounding the agar plate. This led to &hellip; <a href=\"https:\/\/microbiologymatters.com\/?p=1861\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">&#8220;Should anaerobes be cultured from superficial swabs?&#8221;<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false}}},"categories":[4],"tags":[253,251,252],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p37jIp-u1","jetpack-related-posts":[{"id":2731,"url":"https:\/\/microbiologymatters.com\/?p=2731","url_meta":{"origin":1861,"position":0},"title":"&#8220;Should anaerobic culture be performed on superficial swabs ??&#8221;","author":"michael","date":"January 27, 2016","format":false,"excerpt":"This is a (version of) a post I published a couple of years ago, and I am still toying with the idea. Any thoughts or experience with this topic\u00a0would be much appreciated, before I take the plunge! Anaerobic culture has always been difficult in a diagnostic bacteriology laboratory. In the\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":363,"url":"https:\/\/microbiologymatters.com\/?p=363","url_meta":{"origin":1861,"position":1},"title":"Tips for Microbiology Scientists working on Bacteriology Benches. Part III","author":"michael","date":"January 21, 2013","format":false,"excerpt":"\u00a0This is the last part of this \"Tips for Microbiology Scientists\" mini-series. \u00a0 1) Don\u2019t\u00a0\"over-analyse\" anaerobes\u2026 I am not a big fan of identifying all anaerobes isolated, and in particular from superficial sites, where in many cases\u00a0anaerobic culture should not even be undertaken. Whether the clinician includes anaerobic cover when\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1665,"url":"https:\/\/microbiologymatters.com\/?p=1665","url_meta":{"origin":1861,"position":2},"title":"&#8220;The Bottom Line&#8221;","author":"michael","date":"June 5, 2013","format":false,"excerpt":"This post continues the \"merdish\" theme of the previous article. There are two\u00a0of types of microbiology samples in this area\u00a0which I have always had some reservations about as to their usefulness. These are samples from peri-anal abscesses and pilo-nidal sinuses. There a few reasons why I believe that sampling from\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3690,"url":"https:\/\/microbiologymatters.com\/?p=3690","url_meta":{"origin":1861,"position":3},"title":"&#8220;Choosing wisely bacteriology II: Swabs from the peri-anal area&#8221;","author":"michael","date":"December 5, 2017","format":false,"excerpt":"There is so much scope for \"choosing wisely\" in general bacteriology, it is difficult to know where to start... Peri-anal swabs are rarely of value in changing patient management. That is primarily because you are swabbing an incredibly \"dirty\" area in the first place. You can be sure that your\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/microbiologymatters.com\/wp-content\/uploads\/2017\/12\/untitled-300x289.png?resize=350%2C200","width":350,"height":200},"classes":[]},{"id":2172,"url":"https:\/\/microbiologymatters.com\/?p=2172","url_meta":{"origin":1861,"position":4},"title":"&#8220;Twenty Tips for people working on the Bacteriology Benches&#8221;","author":"michael","date":"February 12, 2015","format":false,"excerpt":"Apologies for the paucity of posts recently. Ultra-marathons (click here for update)\u00a0and babies have seen to that! The post below is a combination of posts (with some extra points added)\u00a0published a couple of years ago on this website, and will form the basis for a chapter in my forthcoming book\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":3334,"url":"https:\/\/microbiologymatters.com\/?p=3334","url_meta":{"origin":1861,"position":5},"title":"&#8220;Painting Pictures&#8221;","author":"michael","date":"February 16, 2017","format":false,"excerpt":"A wound swab arrives into the microbiology laboratory.. ...Because there are no clinical details we don't know whether a Gram stain might be worthwhile. Because there are no clinical details, we don't know whether to add anaerobic culture or set up a yeast agar for this\u00a0 swab. Because there are\u2026","rel":"","context":"In &quot;The Art of Microbiology&quot;","block_context":{"text":"The Art of Microbiology","link":"https:\/\/microbiologymatters.com\/?cat=3"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/microbiologymatters.com\/wp-content\/uploads\/2017\/02\/aid206341-728px-Paint-Step-22-300x225.jpg?resize=350%2C200","width":350,"height":200},"classes":[]}],"_links":{"self":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/posts\/1861"}],"collection":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1861"}],"version-history":[{"count":5,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/posts\/1861\/revisions"}],"predecessor-version":[{"id":1868,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/posts\/1861\/revisions\/1868"}],"wp:attachment":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1861"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1861"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1861"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}