{"id":1511,"date":"2013-05-16T14:54:45","date_gmt":"2013-05-16T01:54:45","guid":{"rendered":"http:\/\/microbiologymatters.com\/?page_id=1511"},"modified":"2024-01-12T16:12:44","modified_gmt":"2024-01-12T03:12:44","slug":"mcqs-vzv-laboratory-testing-answers","status":"publish","type":"page","link":"https:\/\/microbiologymatters.com\/?page_id=1511","title":{"rendered":"MCQs: VZV laboratory testing: Answers"},"content":{"rendered":"

1) VZV IgM serology is a sensitive means of picking up acute infection. T\/F<\/span><\/p>\n

False: Several false negative results occur from VZV IgM testing. Many labs no longer offer the assay.<\/span><\/p>\n

2) Most people who don’t have a clinical history of chickenpox have negative VZV IgG on laboratory testing? T\/F<\/span><\/p>\n

False: Most people (80%+) who have no clinical history of chickenpox actually have VZV IgG antibodies on lab testing and are thus immune.<\/span><\/p>\n

3) IgG seroconversion can more reliably be detected after VZV vaccination as opposed to natural infection? T\/F<\/span><\/p>\n

False: Natural infection produces the strongest IgG response. False negative IgG results occasionally produced after vaccination.<\/span><\/p>\n

4) PCR can potentially be used to discriminate between vaccination and wild type strains of VZV? T\/F<\/span><\/p>\n

True: PCR genotyping can potentially discriminate if the assay is designed for this purpose.<\/span><\/p>\n

5) Cotton is a better material than polyester at picking up VZV virus? T\/F<\/span><\/p>\n

False: Polyester is a better material than cotton and is recommended in viral PCR swabs.<\/span><\/p>\n

6) When the rash has crusted over, the PCR is ineffective at picking up VZV? T\/F<\/span><\/p>\n

False: Crusts contain high levels of virus and OCR is usually effective at testing this specimen type.<\/span><\/p>\n

7) PCR is an effective method of distinguishing between a chickenpox and a shingles rash? T\/F<\/span><\/p>\n

False: It is the same virus, so PCR will not discriminate between the two clinical entities.<\/span><\/p>\n

8) VZV Immunofluorescence is commonly used in laboratories to make the diagnosis of chickenpox? T\/F<\/span><\/p>\n

False: It certainly can be used, but in my experience, is certainly not common amongst routine diagnostic laboratories for the diagnosis of VZV.<\/span><\/p>\n

9) VZV is able to be cultured on cell lines? T\/F<\/span><\/p>\n

True: but with poor sensitivity. Viralculture now rarely used to make a diahnosis of VZV in diagnostic laboratories.<\/span><\/p>\n

10) Electron microscopy can discriminate between VZV and Herpes Simplex Virus (HSV)? T\/F<\/span><\/p>\n

False: The two viruses look the same on electron microscopy.<\/span><\/p>\n

 <\/p>\n

Click here<\/span><\/a> to return to the MCQ page<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

1) VZV IgM serology is a sensitive means of picking up acute infection. T\/F False: Several false negative results occur from VZV IgM testing. Many labs no longer offer the assay. 2) Most people who don’t have a clinical history of chickenpox have negative VZV IgG on laboratory testing? T\/F False: Most people (80%+) who … Continue reading MCQs: VZV laboratory testing: Answers<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1509,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P37jIp-on","jetpack-related-posts":[{"id":1509,"url":"https:\/\/microbiologymatters.com\/?page_id=1509","url_meta":{"origin":1511,"position":0},"title":"MCQs: Varicella Zoster Virus (VZV) testing in the laboratory","author":"michael","date":"May 16, 2013","format":false,"excerpt":"Have a go at these 10 True\/False MCQs, then click at the bottom for answers with brief explanations: 1) VZV IgM serology is a sensitive means of picking up acute infection? T\/F 2) Most people who don't have a clinical history of chickenpox have negative VZV IgG on laboratory testing?\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":88,"url":"https:\/\/microbiologymatters.com\/?page_id=88","url_meta":{"origin":1511,"position":1},"title":"MCQs","author":"michael","date":"December 20, 2012","format":false,"excerpt":"\u00a0 Basic Bacterial Cell Biology: Part I Culture Media Neisseria gonorrhoeae Pasteurella multocida PCR: The Basic PCR Reaction. 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Her CMV IgM is low reactive and CMV IgG is moderately reactive. 1) Why is the diagnosis of\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1837,"url":"https:\/\/microbiologymatters.com\/?page_id=1837","url_meta":{"origin":1511,"position":3},"title":"Microbiology Picture Quiz: Number 9: Answers","author":"michael","date":"August 5, 2013","format":false,"excerpt":"This patient presents with unilateral parotid swelling. \u00a0 1) What disease\u00a0connects the\u00a0 presentation with the picture? Mumps. This is an electron micrograph of mumps virus,\u00a0a picture that would not be seen in diagnostic laboratories these days as electron microscopes are alost all now confined to research facilities.\u00a0\u00a0In mumps, parotid swelling\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"\/\/upload.wikimedia.org\/wikipedia\/commons\/5\/54\/Mumps_virus.jpg","width":350,"height":200},"classes":[]},{"id":1777,"url":"https:\/\/microbiologymatters.com\/?page_id=1777","url_meta":{"origin":1511,"position":4},"title":"“Data Interpretation: Case Number 3: Answers”","author":"michael","date":"June 24, 2013","format":false,"excerpt":"A 20 year old presents with a 72 hour history of headache, photophobia and fever. On examination he is alert and orientated and has mild neck stiffness. There\u00a0are no focal neurological signs. He has no significant past medical history. He has no risk factors for HIV nor does he have\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1294,"url":"https:\/\/microbiologymatters.com\/?page_id=1294","url_meta":{"origin":1511,"position":5},"title":"MCQs:Hepatitis B serology: Answers","author":"michael","date":"April 12, 2013","format":false,"excerpt":"1) Most positive HBsAg results picked up on ante-natal screening represent chronic infection? T\/F True, probably more than 95% of such results represent chronic infection, most of whom have probably had the infection for years or even\u00a0since birth. 2) IgM Anti-HBc can be used to differentiate acute from chronic Hepatitis\u2026","rel":"","context":"With 3 comments","block_context":{"text":"With 3 comments","link":"https:\/\/microbiologymatters.com\/?page_id=1294#comments"},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"_links":{"self":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/pages\/1511"}],"collection":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1511"}],"version-history":[{"count":6,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/pages\/1511\/revisions"}],"predecessor-version":[{"id":1526,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/pages\/1511\/revisions\/1526"}],"up":[{"embeddable":true,"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=\/wp\/v2\/pages\/1509"}],"wp:attachment":[{"href":"https:\/\/microbiologymatters.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1511"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}