Category Archives: The Science of Microbiology

“Reinventing yourself”


In the next generation (20-25 years), the diagnostic microbiology laboratory workforce will be decimated worldwide.

If you are a student, they don’t tell you that at the careers fair…

“You sound like exactly the sort of person we are looking for. Come and “train” for four years in a lecture theatre, and then work in a clinical microbiology laboratory, that is if you are lucky enough to get a job. Unless your Mum and Dad are well off, you will accrue a hefty debt which will likely take you a couple of decades to pay off. However in 20 years time your job will probably not even exist…”

Am I being too harsh?

In terms of general culture based bacteriology, most of it will have gone molecular. Whatever is left of it will be automated, not just partially automated as with the current Kiestra TLA system, but completely automated to include plate interpretation, colony picking, identification, antibiotic susceptibility testing and rule based signout. The whole works…

Most microbiology samples will never touch a laboratory worker’s hands.

Molecular testing will have increased, but on highly automated platforms, processing high volumes of work, with minimum manual input.

Our work will be reduced to oddities and troubleshooting. I would even chance to say that there will be just as many engineers as microbiologists on the laboratory floor.

And then I look at the core components of my own job as a clinical microbiologist…

  • Authorising important results:- This will be done automatically using sophisticated rules based computer algorithms. And they will do it much better than I can.
  • Giving antibiotic advice:- Decision support apps downloaded on clinicians’ smartphones will do this more sensibly than me.
  • Laboratory Management:- I fear there will be nobody left in the lab to manage…
  • Demand management:- Although the process of demand management will be performed by software algorithms, there might still be a little work left for me regarding the initiation and governance of such projects.
  • Anti-microbial stewardship:- Anti-microbial resistance is not going away anytime soon so there may be a continuing role in the governance of such programmes. But the nuts and bolts will be highly automated and app-based.

So I am not overly optimistic about my own long term future. No one is immune…

It is no accident that during conferences I heavily focus  on presentations in molecular diagnostics and demand management. That should help in the short term at least in securing my usefulness. However it is entirely possible I will need to retrain in something completely different before I am done.

I know my job description as a clinical microbiologist will change out of all recognition before I retire. It is not impossible that clinical microbiology as a career entity will cease to exist altogether.

We need to be constantly looking at what we do today, then imagining what we will potentially be doing tomorrow, and preparing for it as best we can…

Michael

“Reflex Testing: A Hot Potato”

A reflex test in the laboratory setting is most simply defined as a test that the laboratory automatically adds on based on the result of the initial requested test, in order to optimise the diagnostic process.

Sounds like a no-brainer…

For example one might add a Hepatitis C viral load test to a sample which is positive for antibodies to Hepatitis C. Or one could add cytomegalovirus (CMV) serology to a sample which is negative for Epstein Barr virus (EBV), but with clinical and blood count parameters consistent with infectious mononucleosis. Within molecular testing, an example might be reflex testing for trichomonas in a patient who tests positive for either chlamydia or gonorrhoea. There is not so much potential for reflex testing in classical culture based bacteriology, unless of course you consider antibiotic susceptibility testing to be a reflex test, which of course it is to a certain extent. And once you delve into the field of immunology, the potential for reflex testing is massive…

The potential list of reflex tests for a clinical laboratory is actually extremely long.

So what reflex testing should be performed by the laboratory? This is actually a bit of a political hot potato.

Because everyone has their different agendas on reflex testing:

The laboratory’s view of reflex testing will depend to an extent on how they are funded. If they are funded on a fee per service/test model, they will welcome reflex testing with open arms, and utilise every opportunity to implement and encourage it. More tests equals more revenue.

If however the laboratory is funded on a “capped budget”, they will shy away from reflex testing. Extra tests in this setting means extra expenditure.

With regards to the laboratory funders, occasionally they will see that reflex testing might save them money in the long run through decreased hospital care etc. In general however, funders aren’t big fans of reflex testing, as it essentially means extra tests to fund (see idealism and realism below)

Requestors generally like the idea of reflex testing. It prevents a further consultation, further sampling etc. Anything to make the system run smoother. Occasionally though requestors get annoyed at getting a result on a test which they have not requested.

For patients also, reflex testing is generally a good thing. One must always be wary however of the consent process. Will the reflex test be included in the initial consent for laboratory testing? Personally I think that consent for laboratory testing should be on the premise that the laboratory will do “whatever it takes” to diagnose the cause of the patient’s symptoms. We don’t do test consenting very well as a rule…

Reflex testing demonstrates the difference between idealism and realism. For example 100 patients have a positive test X necessitating test Y. If test Y was reflexed by the laboratory then all 100 patients  would receive test Y. If the undertaking of test Y depended on the requestor reviewing the result of test X and actively requesting test Y, only a proportion of the 100 patients would end up getting test Y done. This is the real world.

The clinical value of reflex testing is also a continuous spectrum. For some reflex tests, the clinical value of adding on the test automatically is unequivocally high. For others the clinical value (and cost-effectiveness) is much more debatable.

Because it is such a political area, I think it is important that none of the groups above have undue influence in what reflex testing is done. Ideally a completely independent panel should set the criteria for reflex testing, and preferably on a national basis.

“No science is immune to the infection of politics and the corruption of power.”  (Jacob Bronowski)

Michael

 

Plasmids and Team Players

Let’s say you have a problem at your hospital with carbapenemases.

One of the obvious solutions would be to reduce the use of carbapenems in order to reduce the selection pressure.

However even if you stopped carbapenem usage altogether the carbapenemases would not necessarily disappear…

This is because carbapenemases are often plasmid borne, and there are often antibiotic resistance genes for other antibiotics, e.g. A, B & C sitting on the same plasmid.

As long as the (high) usage of antibiotics A, B & C continued then the selection pressure would favour plasmid retention in the bacterium, and thus allowing persistence of the carbapenemase.

Selection pressure by proxy.

Are we all doomed?

Not necessarily…

A gene expressing one antimicrobial resistance determinant comes at an energy cost to a bacterial cell. Plasmids expressing multiple resistance genes come at even more energy cost to the cell. You can be sure if it did not need the plasmid to ensure its survival, it would be mercilessly dumped, and probably sooner rather than later.

Therefore even a modest reduction in carbapenem usage, along with a reduction in antibiotics A, B & C may go a long way to solving your problem.

Advances in molecular methods and whole genome sequencing over the next decade will mean that it will become much easier to work out exactly which resistance genes are contained in the plasmids circulating in our local hospitals, and anti-microbial stewardship can thus be optimised accordingly.

Sounds space age?

Not really, we just need to be aware that resistant bacteria are very smart in an evolutionary sense, and we need to stay alert, and not give them the niches they are looking for…

Michael

Illustration courtesy of www.biologyfun.blogspot.co.nz